International Journal of Innovative Research and Knowledge

Volume-3 Issue-12, December 2018

Title: Assessing Genetic diversity of an endangered medicinal plant, Strychnos henningsii (Gilg.) in Nine Populations in Kenyan counties as revealed by ISSR Markers



Strychnos henningsii (Gilg.) is an endangered medicinal plant in Kenya due to over exploitation for medicinal purposes. To understand the levels of genetic variation across populations and geographical regions of this species, we aimed at assessing the genetic diversity in nine Kenyan populations of Strychnos henningsii using inter-simple sequence repeat (ISSR) Markers. A total of 270 samples from nine populations of S. henningsii were collected. The genetic variation within and among populations were evaluated using inter-simple sequence repeat (ISSR). A total of 96 loci were revealed by ISSR primers all of which were polymorphic. These markers revealed that Ngong population was the most polymorphic with 51 (53.12%) polymorphic loci and Baringo as the least polymorphic with 28 (29.17%) polymorphic loci detected. Thirteen population specific loci were also revealed by ISSR markers which might have contributed to specific population traits. A higher molecular variance was showed among populations 58% (p>0.001) than 41% within populations. According to Nei’s unbiased genetic distance matrix the most genetically close populations were Taveta and Marsabit with the highest genetic identity (0.8803) and the lowest genetic distance (0.1275) between Jilore and Baringo. Clustering analysis based on Nei’s genetic distance grouped the nine population into three; Cluster I consisted of three populations namely Kitui, Ngong and Jilore. Cluster II consisted of five populations (Marsabit, Taveta, Nyeri, Narok and Karura) and cluster III consisted of Baringo population. These results were also supported by principal coordinate analysis. The present findings indicated a low genetic variation between and among the existing genotypes of S. henningsii. This implies that the conservation efforts should aim to preserve all the existing populations of this threatened plant species along with extensive domestication for commercial purpose.

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ISSN: 2213-1356

Publisher: Scholar Touch Publishers

Area/Scope: Business, Economics & Management; Social Science, Literature, Arts & Humanities; Engineering & Technology; Life Science & Physical Science, Health & Medical Science

Frequency: Monthly

Format: Online & Print

Language: English

Review Process: Double Blinded

Access: Open Access